Figure 1. Western blot analysis of GLOD5 using anti-GLOD5 antibody (A19277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLOD5 antigen affinity purified polyclonal antibody (A19277) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLOD5 at approximately 16 kDa. The expected band size for GLOD5 is at 18 kDa.
Figure 1. Western blot analysis of GLOD5 using anti-GLOD5 antibody (A19277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLOD5 antigen affinity purified polyclonal antibody (A19277) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLOD5 at approximately 16 kDa. The expected band size for GLOD5 is at 18 kDa.
Anti-GLOD5 Antibody Picoband(r)
A19277-IFLUOR647
TargetGLOD5
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-GLOD5 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID392465
- Target nameGLOD5
- Target descriptionglyoxalase domain containing 5
- Target synonymsglyoxalase domain-containing protein 5
- Protein IDA6NK44
- Protein NameGlyoxalase domain-containing protein 5
- Scientific DescriptionBoster Bio Anti-GLOD5 Antibody Picoband® catalog # A19277. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203