IHC analysis of GLP1 using anti-GLP1 antibody (PB9705). GLP1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of GLP1 using anti-GLP1 antibody (PB9705). GLP1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-GLP1/GCG Antibody
PB9705
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetGCG
Overview
- SupplierBoster Bio
- Product NameAnti-GLP1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2641
- Target nameGCG
- Target descriptionglucagon
- Target synonymsglicentin-related polypeptide; GLP1; GLP-1; GLP2; glucagon-like peptide 1; glucagon-like peptide 2; GRPP; preproglucagon; pro-glucagon
- HostRabbit
- IsotypeIgG
- Protein IDP01275
- Protein NameGlucagon
- Scientific DescriptionBoster Bio Anti-GLP1/GCG Antibody Picoband® catalog # PB9705. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Glutathione prevents chronic oscillating glucose intake-induced beta-cell dedifferentiation and failure. Zhang J et al., 2019 Apr 11, Cell Death DisRead more
- The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlying signaling mechanisms in a rat model of diabetes mellitus. Zhang Y et al., 2016 Oct 10, Sci RepRead more