Figure 1. Western blot analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: PANC Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: HT1080 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A2 antigen affinity purified polyclonal antibody (Catalog # PB9436) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A2 at approximately 50 kDa. The expected band size for SLC2A2 is at 57 kDa.
Figure 1. Western blot analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: PANC Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: HT1080 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A2 antigen affinity purified polyclonal antibody (Catalog # PB9436) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A2 at approximately 50 kDa. The expected band size for SLC2A2 is at 57 kDa.
Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband(r)
PB9436-PE
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC2A2
Overview
- SupplierBoster Bio
- Product NameAnti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for SLC2A2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID6514
- Target nameSLC2A2
- Target descriptionsolute carrier family 2 member 2
- Target synonymsglucose transporter type 2, liver; GLUT2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2
- HostRabbit
- IsotypeIgG
- Protein IDP11168
- Protein NameSolute carrier family 2, facilitated glucose transporter member 2
- Scientific DescriptionBoster Bio Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® catalog # PB9436. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203