Figure 1. Western blot analysis of GRIA3 using anti-GRIA3 antibody (PB9206). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA3 antigen affinity purified polyclonal antibody (Catalog # PB9206) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA3 at approximately 101 kDa. The expected band size for GRIA3 is at 101 kDa.
Figure 1. Western blot analysis of GRIA3 using anti-GRIA3 antibody (PB9206). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA3 antigen affinity purified polyclonal antibody (Catalog # PB9206) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA3 at approximately 101 kDa. The expected band size for GRIA3 is at 101 kDa.
Anti-Glutamate receptor 3/GRIA3 Antibody Picoband(r)
PB9206-IFLUOR647
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRIA3
Overview
- SupplierBoster Bio
- Product NameAnti-Glutamate receptor 3/GRIA3 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for GRIA3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID2892
- Target nameGRIA3
- Target descriptionglutamate ionotropic receptor AMPA type subunit 3
- Target synonymsAMPA-selective glutamate receptor 3; dJ1171F9.1; GluA3; GLUR3; gluR-3; GLURC; GLUR-C; GLUR-K3; glutamate receptor 3; glutamate receptor C; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; MRX94; MRXSW
- HostRabbit
- IsotypeIgG
- Protein IDP42263
- Protein NameGlutamate receptor 3
- Scientific DescriptionBoster Bio Anti-Glutamate receptor 3/GRIA3 Antibody Picoband® catalog # PB9206. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203