Anti-Glycoprotein E2 [AP33]

Absolute Antibody

Anti-Glycoprotein E2 [AP33]

9

This antibody recognizes a linear epitope comprising residues 412 to 423 of HCV envelope glycoprotein E2. The hepatitis C virus is the cause of hepatitis C and some cancers such as liver cancer (hepatocellular carcinoma, abbreviated HCC) and lymphomas in

The binding of AP33 inhibits the interaction between CD81 and a range of presentations of E2, including soluble E2, E1E2, and virus-like particles. To asses and the reactivity and neutralizing capacity of antisera and monoclonal antibodies, proteins from HEK293T cells cotransfected with a plasmid containing HCV genotype 1a strain H77-derived full-length E1E2 were immunoprecipitated using the mouse version of this antibody. Further on the same HEK293T cells an ELISA was performed using the mouse version of this antibody. Finally a neutralization assay was performed by incubating huh-7 cells with HCVpp in the presence of the mouse version of this antibody and then measuring the fluorescence using flow cytometry (Owsianka et al, 2005; pmid:16103160). To measure the relative binding affinity of the mouse version of this antibody to HCV E1E2 glycoproteins, a cell lysate-based ELISA was performed. Further HCV pseudo particles (HCVpp) and cell-culture-derived HCV (HCVcc) neutralization assays were performed (Desombere et al, 2016; pmid:26710081). To determine the reactivity of selected phage with the selecting antibody, a phage capture enzyme immunoassay was performed. For this assay the mouse version of this antibody was used. Further to detect MAb binding to E2 glycoprotein, an ELISA was performed on transfected HEK293FT cells using the mouse version of this antibody (Tarr et al, 2006; pmid:16496330). Binding of the mouse version of this antibody was assessed in the presence of the peptide-reactive serum, and HCV-positive, peptide-unreactive serum and normal human serum. Further to determine the neutralization ability of this antibody a neutralization assay was performed on huh-7 cells and HEK293T cells infected with HCVpps (Tarr et al, 2007; pmid:17947521).

Functional Assay, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

AP33

Rabbit

IgG

Genome polyprotein

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203