Anti-Glycoprotein GPIIb/IIIa complex [314.3]

Absolute Antibody

Anti-Glycoprotein GPIIb/IIIa complex [314.3]

9

The antibody recognizes the N terminus of the GPIIb beta propeller domain only when soluble quinine is present.

The antibody reacted with normal human platelets in the presence of quinine, as shown by flow cytometry. The reactions of the antibody with CHO cells expressing all-human GPIIb/IIIa (H/H), all-rat GPIIb/IIIa (R/R), or human/rat mixed GPIIb/IIIa integrins (H/R, R/H) were studied by flow cytometry. Quinine dependent antibody binding occurred with constructs H/H and H/R, but not with R/R or R/H (Bougie et al., 2009; PMID: 18948570). The crystal structures of the Fab fragment and its complex with quinine were determined. Quinine induced structural modifications of the Fab fragment; the authors propose that quinine remodels the Fab paratope and enables binding to the platelet GPIIb/IIIa. The monovalent KD values for quinine binding to the IgG fragment measured was 24 nM (Zhu et al., 2015; PMID: 26282540).

Flow Cytometry, Other Application

Research Use Only

Monoclonal

314.3

ITGB3

integrin subunit beta 3

antigen CD61; BDPLT16; BDPLT2; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2; integrin beta 3; integrin beta chain, beta 3; integrin beta-3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); platelet membrane glycoprotein IIIa

Rabbit

IgG

Integrin beta-3

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203