Anti-Glycoprotein H [A13-d6.3]

Absolute Antibody

Anti-Glycoprotein H [A13-d6.3]

9

d6.3 Fab fragment binds to the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107-639) which is the membrane-proximal end of the protein in the deltagL-Pass PrV variant of PrV. The antibody contacts residues 332-373 (peripheral helices alpha6

d6.3 neutralizes the deltagL-Pass PrV variant of PrV and can stain deltagL-Pass PrV variant but not the wild-type PrV by immunostaining. The epitope of d6.3 is not blocked by the formation of the gH/gL heterodimer. The d6.3Fab fragment (obtained through papain digestion) can be complexed with the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107-639) for structural studies by crystallography.

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

A13-d6.3

Mouse

IgG2b

Virus

-20°C,2°C to 8°C

12352203