Anti-Glypican 3 [7H1]

Absolute Antibody

Anti-Glypican 3 [7H1]

7

This antibody binds an epitope between amino acids 25 to 137 of the human glypican 3 (GPC3). This antibody cross reacts with cynomolgus monkey, rat and mouse GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphat

This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. This antibody strongly reacts with hepatic cancer tissue microarray, JHH cells, HepG2 cells, and cells stably transfected with GPC3 in immunohistochemistry. This antibody also detects human, cynomolgus monkey, rat, and mouse GPC3 by western blot. This antibody was also used in the generation of an antibody drug conjugate with maleimide acetal PNU-159682 or monomethyl disulfide N10-linked PBD. The ADCC activity of the antibody drug conjugate was investigated using a human HepG2 X1 and JHH7 xenograft model and substantial tumor growth inhibition was achieved with 7H1-disulfide-PBD (US9926377).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry

FC; ELISA; IHC; WB

Research Use Only

Monoclonal

7H1

GPC3

glypican 3

DGSX; glypican proteoglycan 3; glypican-3; GTR2-2; heparan sulphate proteoglycan; intestinal protein OCI-5; MXR7; OCI-5; SDYS; secreted glypican-3; SGB; SGBS; SGBS1

Rabbit

IgG

Glypican-3

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Monkey, Mouse, Rat

Human, cynomolgus monkey, rat, mouse

The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).

-20°C,2°C to 8°C

12352203