Anti-Glypican 3 [AB13E3]

Absolute Antibody

Anti-Glypican 3 [AB13E3]

7

This antibody binds human glypican 3 (GPC3).Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positive

The binding specificity of this antibody for human glypican 3 was determined using ELISA. The original IgG1 version of this antibody binds human GPC3 with an affinity of Kd = 2.84 pM. This antibody can bind GPC3 expressed on the surface of PLC/PRF/5 and Huh7 cells in an in vitro binding assay. This antibody also positively shows ADCC activity on PLC/PRF/5 cells expressing glypican 3 (CN109988240).

Functional Assay, ELISA

ELISA; functional assay

Research Use Only

Monoclonal

AB13E3

GPC3

glypican 3

DGSX; glypican proteoglycan 3; glypican-3; GTR2-2; heparan sulphate proteoglycan; intestinal protein OCI-5; MXR7; OCI-5; SDYS; secreted glypican-3; SGB; SGBS; SGBS1

Rabbit

IgG

Glypican-3

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The parental mouse antibody GPC3#4 was generated by immunizing BALB/c mice with 50mug of purified human GPC-3 antigen emulsified in Freund's complete adjuvant. This original antibody was generated be grafting CDRs of the parental mouse antibody onto human framework region.

-20°C,2°C to 8°C

12352203