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Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (A06975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG2 antigen affinity purified polyclonal antibody (Catalog # A06975-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 12KD.
Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (A06975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG2 antigen affinity purified polyclonal antibody (Catalog # A06975-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 12KD.
Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (A06975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG2 antigen affinity purified polyclonal antibody (Catalog # A06975-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 12KD.

Anti-GNG2 Antibody Picoband(r)

A06975-1-PE
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGNG2
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GNG2 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    RPE
  • Gene ID54331
  • Target name
    GNG2
  • Target description
    G protein subunit gamma 2
  • Target synonyms
    g gamma-I; guanine nucleotide binding protein (G protein), gamma 2; guanine nucleotide binding protein gamma 2; guanine nucleotide-binding protein G(I)/G(O) gamma-2 subunit; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP59768
  • Protein Name
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
  • Scientific Description
    Boster Bio Anti-GNG2 Antibody Picoband® catalog # A06975-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203