
Figure 1. Western blot analysis of Gp2 using anti-Gp2 antibody (A06630-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gp2 antigen affinity purified polyclonal antibody (Catalog # A06630-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gp2 at approximately 55KD. The expected band size for Gp2 is at 59KD.
Anti-GP2 Picoband(r) Antibody

A06630-1-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetGp2
Overview
- SupplierBoster Bio
- Product NameAnti-GP2 Picoband(r) Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID67133
- Target nameGp2
- Target descriptionglycoprotein 2 (zymogen granule membrane)
- Target synonyms2310037I18Rik; AV060639; pancreatic secretory granule membrane major glycoprotein GP2; pancreatic zymogen granule membrane protein GP-2
- HostRabbit
- IsotypeIgG
- Protein IDQ9D733
- Protein NamePancreatic secretory granule membrane major glycoprotein GP2
- Scientific DescriptionBoster Bio Anti-GP2 Picoband® Antibody catalog # A06630-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203