Figure 2. IHC analysis of GRK5 using anti-GRK5 antibody (PB9708). GRK5 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-GRK5 Antibody (PB9708) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of GRK5 using anti-GRK5 antibody (PB9708). GRK5 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-GRK5 Antibody (PB9708) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-GRK5 Antibody Picoband(r)
PB9708
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetGRK5
Overview
- SupplierBoster Bio
- Product NameAnti-GRK5 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2869
- Target nameGRK5
- Target descriptionG protein-coupled receptor kinase 5
- Target synonymsFP2025; G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5; GPRK5
- HostRabbit
- IsotypeIgG
- Protein IDP34947
- Protein NameG protein-coupled receptor kinase 5
- Scientific DescriptionBoster Bio Anti-GRK5 Antibody Picoband® catalog # PB9708. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityChicken, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203