
Figure 1. Western blot analysis of H-ERG/KCNH2 using anti-H-ERG/KCNH2 antibody (A00781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissuelysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H-ERG/KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H-ERG/KCNH2 at approximately 130 kDa. The expected band size for H-ERG/KCNH2 is at 127 kDa.
Anti-H-ERG/KCNH2 Antibody Picoband(r)
A00781-3-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNH2
Overview
- SupplierBoster Bio
- Product NameAnti-H-ERG/KCNH2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID3757
- Target nameKCNH2
- Target descriptionpotassium voltage-gated channel subfamily H member 2
- Target synonymseag homolog; eag-related protein 1; ERG1; ERG-1; ether-a-go-go-related gene potassium channel 1; ether-a-go-go-related potassium channel protein; ether-a-go-go-related protein 1; HERG; H-ERG; HERG1; human ether-a-go-go-related; Kv11.1; long QT syndrome type 2; LQT2; potassium channel, voltage gated eag related subfamily H, member 2; potassium voltage-gated channel subfamily H member 2; potassium voltage-gated channel, subfamily H (eag-related), member 2; SQT1; voltage-gated potassium channel subunit Kv11.1
- HostRabbit
- IsotypeIgG
- Protein IDQ12809
- Protein NamePotassium voltage-gated channel subfamily H member 2
- Scientific DescriptionBoster Bio Anti-H-ERG/KCNH2 Antibody Picoband® catalog # A00781-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203