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Figure 1. Western blot analysis of H-ERG/KCNH2 using anti-H-ERG/KCNH2 antibody (A00781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissuelysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H-ERG/KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H-ERG/KCNH2 at approximately 130 kDa. The expected band size for H-ERG/KCNH2 is at 127 kDa.
Figure 1. Western blot analysis of H-ERG/KCNH2 using anti-H-ERG/KCNH2 antibody (A00781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissuelysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H-ERG/KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H-ERG/KCNH2 at approximately 130 kDa. The expected band size for H-ERG/KCNH2 is at 127 kDa.
Figure 1. Western blot analysis of H-ERG/KCNH2 using anti-H-ERG/KCNH2 antibody (A00781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissuelysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H-ERG/KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H-ERG/KCNH2 at approximately 130 kDa. The expected band size for H-ERG/KCNH2 is at 127 kDa.

Anti-H-ERG/KCNH2 Antibody Picoband(r)

A00781-3-IFLUOR647
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNH2
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-H-ERG/KCNH2 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID3757
  • Target name
    KCNH2
  • Target description
    potassium voltage-gated channel subfamily H member 2
  • Target synonyms
    eag homolog; eag-related protein 1; ERG1; ERG-1; ether-a-go-go-related gene potassium channel 1; ether-a-go-go-related potassium channel protein; ether-a-go-go-related protein 1; HERG; H-ERG; HERG1; human ether-a-go-go-related; Kv11.1; long QT syndrome type 2; LQT2; potassium channel, voltage gated eag related subfamily H, member 2; potassium voltage-gated channel subfamily H member 2; potassium voltage-gated channel, subfamily H (eag-related), member 2; SQT1; voltage-gated potassium channel subunit Kv11.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12809
  • Protein Name
    Potassium voltage-gated channel subfamily H member 2
  • Scientific Description
    Boster Bio Anti-H-ERG/KCNH2 Antibody Picoband® catalog # A00781-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203