Anti-HA [AV.C4]

Absolute Antibody

Anti-HA [AV.C4]

9

This antibody binds the hemaglutinin of the H5N1 avian influenza virus.

The specificity of this scFv antibody for binding H5 type of hemaglutinin was determined by ELISA. AV.C4 binds a conserved epitope shared by three different virus strains belonging to the H5 subtype, i.e. the homologous strain A/Vietnam/ 1194/04 (H5N1), and two viruses isolated form wild birds, A/Nammalr/Italy/80/93 (H5N2) and A/Mallard/Italy/208/00 (H5N3). This antibody does not cross react with H1N1 influenza virus. scFv clone AV.C4 exerted a significant inhibition of the H5N1 A/Vietnam/1194/2004 virus infection in a pseudotype-based neutralization assay. This antibody was capable of detecting a 70kDa protein corresponding to the uncleaved HA protein in a western blot assay (PMID: 19481117).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

AV.C4

Rabbit

IgG

Hemagglutinin

This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203