Anti-HA cleavage site [A3]

Absolute Antibody

Anti-HA cleavage site [A3]

9

The antibody specifically recognizes the sequence (NS)PQRER observed at the cleavage site of the hemagglutinin protein of the H5N1 avian influenza virus. The antibody does not cross-react with the HA protein of H1N1 or H7N7 strain.

The specificity of this antibody (Fab) was confirmed by ELISA. The epitope mapping of this antibody was done using ELISA. The binding affinity of the Fab fragment towards the cleavage site peptide of HA derived from the HPAI H5N1 virus was found to be Kd= 0,80 nM using surface plasmon resonance. The clone was used for detection of the cleavage site peptide on an open sandwich ELISA, reaching the lower limit of detection (LOD) in the range of 1 to 10 ng/ml. This antibody was also used in the immunofluorescent staining of Madin-Darby canine kidney cells infected with HPAI H5N1 (Dong et al, 2013; pmid:23577205).

ImmunoFluorescence, ELISA, Other Application

Research Use Only

Monoclonal

A3

Human

IgM

Hemagglutinin

This chimeric human antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203