Anti-HA [Mab 3.1]

Absolute Antibody

Anti-HA [Mab 3.1]

7

The antibody recognizes the conserved heterosubtypic epitope in the stem of influenza A virus hemagglutinin. The epitope consists of residues from the N- and C-terminal regions of HA1 (38, 40 to 42, 289 to 293, and 318) and the N-terminal portion of HA2 (

In ELISAs, the Fab fragment was found to bind to recombinant HA protein. The Fab fragment neutralized viruses of the H1, H2, H5, and H6 subtypes at half-maximal inhibitory concentrations in the ug/ml range. Passive immunization with the Fab fragment protected mice from a lethal challenge with A/Puerto Rico/8/1934(H1N1) virus. The crystal structure of the antibody in complex with HA from a pandemic H1N1 influenza virus was determined.

ELISA, Neutralisation/Blocking, Other Application

ELISA; neutralization; in vivo; crystallization

Research Use Only

Monoclonal

Mab 3.1

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Virus

Influenza A (H1N1)

A Fab phage display library was prepared from mature B cells of a healthy donor and the original antibody was isolated by panning against HA from A/Japan/305/1957 (H2N2) virus.

-20°C,2°C to 8°C

12352203