Anti-Hairpin DNA [DNA-1]

Absolute Antibody

Anti-Hairpin DNA [DNA-1]

7

The original format of this antibody (Fab) was generated and characterized. It was shown to bind DNA in an IP assay. and its binding affinity was determined to be (4.4+/-2.4) x 10-8 M (Calcutt et al., 1993; PMID: 7506692). Fluorescence titration of the antibody with dT5 was performed, and a Kd of ~27 microM was measured (Komissarov et al., 1996; PMID: 8647821). The crystal structure of the Fab fragment in complex with dT5 was determined (Tanner et al., 2001; PMID: 11733999). Isothermal titration calorimetry experiments were used to measure the apparent affinity of the antibody for dT5. The apparent association constant was KA = 6.0 x 104 M-1. Furthermore, the binding of dT5 to the antibody was exothermic, with an apparent deltaHcal under solution conditions of -16.8 kcal/mol (Schuermann et al., 2004; PMID: 15340914). The crystal structure of the Fab version of this antibody was characterized in complex with a DNA ligand corresponding to nucleotides 5-14 of LIG1-17 (LIG5-14) (Ou et al., 2007; PMID: 18028946).

ImmunoPrecipitation, ELISA, Other Application

Research Use Only

-20°C,2°C to 8°C

12352203