Anti-HBsAg [F124]

Absolute Antibody

Anti-HBsAg [F124 scFv]

7

F124 was used as a solid-phase coating for RIAs detecting HBV particles, resulting in particle immobilisation by binding the pre-S2 epitope of the surface antigen (Budkowska et al, 1986). An scFv-formatted version of F124, comprising the same VH and VL sequences, showed dose-dependent binding to, and competitive blocking of IgG and Fab against, r-HBsAg in an ELISA (Passafiume et al, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations.

Western Blot, ELISA, Neutralisation/Blocking, RadioImmunoAssay

ELISA

Research Use Only

-20°C,2°C to 8°C

12352203