Anti-HCMV [8F9]

Absolute Antibody

Anti-HCMV [8F9]

9

This antibody recognizes and binds a short peptide sequence (ETIYNTTLKY) or the AD-2S1 epitope tag of HCMV envelope glycoprotein B.

This antibody binds a 10aa linear epitope AD-2S1 within the extracellular domain of the human cytomegalovirus glycoprotein B and neutralizes the HCMV infection. Reactivity of this antibody to AD-2S1 was confirmed by ELISA. The ability of the antibody to block the infection of human foreskin fibroblasts by HCMV strain AD169 was done by serially diluting the the antibody starting at 15 microg/ml to determine the concentration required for 50% inhibition of infectivity (IC50).The IC50 for the 8F9 IgG1 was ~0.8 microg/ml. It was further confirmed that only the unmutated, primary Ig gH(M):gL(J1) that included the junctional motif seen in 8F9 exhibited detectable binding to gB (PMID:15814702). Both N-terminal and C-terminal AD-2S1 epitope-tagged human GRB2 was detected by 8F9 in a western blot assay. 8F9 was capable of precipitating 80% of the N-terminal and C-terminal AD-2S1 tagged GRB2 from the cell lysates after immunoprecipitation. Immunofluorescence staining was used to detect cells expressing the AD-2S1 tagged GRB2 (PMID:16919678).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

8F9

Human

IgG1

Envelope glycoprotein B

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203