Figure 1. Western blot analysis of HDHD5 using anti-HDHD5 antibody (A31869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD5 antigen affinity purified polyclonal antibody (Catalog # A31869-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDHD5 at approximately 50 kDa. The expected band size for HDHD5 is at 46 kDa.
Figure 1. Western blot analysis of HDHD5 using anti-HDHD5 antibody (A31869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD5 antigen affinity purified polyclonal antibody (Catalog # A31869-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDHD5 at approximately 50 kDa. The expected band size for HDHD5 is at 46 kDa.
Anti-HDHD5 Antibody Picoband(r)
A31869-1-APC
TargetHDHD5
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-HDHD5 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID27440
- Target nameHDHD5
- Target descriptionhaloacid dehalogenase like hydrolase domain containing 5
- Target synonymscat eye syndrome chromosome region, candidate 5; cat eye syndrome critical region protein 5; CECR5; haloacid dehalogenase-like hydrolase domain-containing 5
- Protein IDQ9BXW7
- Protein NameHaloacid dehalogenase-like hydrolase domain-containing 5
- Scientific DescriptionBoster Bio Anti-HDHD5 Antibody Picoband® catalog # A31869-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203