Anti-Heat-labile enterotoxin B chain [anti-CTP3-TE33], Rabbit IgG, kappa

Absolute Antibody

Anti-Heat-labile enterotoxin B chain [anti-CTP3-TE33], Rabbit IgG, kappa

9

The epitope of this antibody is the sequence: VEVPGSQHIDSQKKA from Cholera toxin peptide 3 (CTP3)

The original antibody was used for fluorescence quenching on DNP-CTP3. This experiment was performed to determine the binding affinity of this antibody, which is 1.2 x 10^6 M^-1. The antibody was used for a radioimmunoassay on cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli isolated from humans. The antibody bound to both antigens. The antibody was used for a western blot on cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli isolated from humans. Which showed the same results as the radioimmunoassay (Anglister et al., 1988; PMID:2450576). The structure of this antibody was determined using NMR measurements (Zilber et al., 1990; PMID:2271636). The structure of this antibody was determined using X-ray crystallography (Shoham, 1993; PMID:7690406). This antibody was used for Phage-colony dot immunoblotting. This antibody was used for an ELISA on synthetic peptide VEVPGSQHIDSQKKA. Further the antibody was used for surface plasmon resonance on the same peptide (Otte et al., 2006; PMID:16273596).

Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

anti-CTP3-TE33

Rabbit

IgG

Heat-labile enterotoxin B chain

Bacteria

-20°C,2°C to 8°C

12352203