Anti-Hepcidin-25 [19D12]

Absolute Antibody

Anti-Hepcidin-25 [19D12]

9

This antibody is specific for amino acids DTHFPICIFCCGCCHRSKCGMCCKT of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightl

The original IgG antibody binds human hepcidin-25 with a binding affinity of Kd= 340 pM (PMID: 23945155). This antibody can be used in combination with another anti-hepcidin antibody 19D12 for detection of human hepcidin in human serum using a sandwich ELISA. 19D12 was used as a capture antibody and 23F11 was used for detection. The limit of sensitivity for the assay was 30 pg/mL (PMID: 23945155). This antibody was also used for immunohistochemical analysis of formalin-fixed, paraffin-embedded sections of prostrate cancer tissues (PMID: 25858146). This antibody was also used to block hepcidin in a study to confirm the functionality of hepcidin in breast tumor spheroids. As expected, blockade of hepcidin increased ferroprotein, indicating that hepcidin synthesized by these cells functions in an autocrine fashion and exerts its expected biological activity (PMID: 29695834).

ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

19D12

HAMP

hepcidin antimicrobial peptide

HEPC; hepcidin; hepcidin preproprotein; HFE2B; LEAP1; liver-expressed antimicrobial peptide 1; PLTR; putative liver tumor regressor

Mouse

IgG2b

Hepcidin

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203