Anti-Hepcidin-25 [AN-LP1]

Absolute Antibody

Anti-Hepcidin-25 [AN-LP1]

9

This antibody is specific for amino acids DTHFPICIFCCGCCHRSKCGMCCKT of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightl

The original mouse IgG1 antibody binds human hepcidin-25 with a binding affinity of Kd= 99 pM. The binding characterization of this antibody to synthetic human hepcidin-25 was done using ELISA. This antibody can be used for the identification of synthetic human hepcidin or human hepcidin from the sera of patients with an inflammatory disease using dot blot and western blot. This antibody was also used for the immunohistochemical analysis of paraffine-embedded human liver biopsies. The biological activity of this antibody was tested by incubating this antibody with macrophages from J774 murine cell line, and it was reported that this antibody induces ferroprotein degradation (US8487081).

Functional Assay, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

AN-LP1

HAMP

hepcidin antimicrobial peptide

HEPC; hepcidin; hepcidin preproprotein; HFE2B; LEAP1; liver-expressed antimicrobial peptide 1; PLTR; putative liver tumor regressor

Mouse

IgG1

Hepcidin

Human

-20°C,2°C to 8°C

12352203