Anti-HER1 [048-006]

Absolute Antibody

Anti-HER1 [048-006]

9

The antibody is specific for HER1. HER1 is a receptor tyrosine kinase binding ligands of the EGF family. It actives several signaling cascades to convert extracellular cues into appropriate cellular responses.

The antibody was able to inhibit the phosphorylation signal of HER1 in three kinds of cells (renal cell carcinoma (CCF-RC1, Caki-1) and epidermoid cancer (A431)) in a concentration-dependent manner, as shown by western blot analysis. The antibody could inhibit the binding to EGF, using A431 cells. The binding affinity of the original format of the antibody to HER1 was measured by surface plasmon resonance (Kd= 0.48 pM). The IgG1 format of the antibody exhibited ADCC activity in the cell line in which HER1 is expressed (CCF-RC-1, NCI-H1373, HT-29, ACHN and A-431 cell lines). The IgG1 format of the antibody was used in in vivo antitumor experiments. Human lung cancer cells H1373 cells or epidermoid tumor A-431 were transplanted into a mouse so as to produce a tumor and, the antibody was administrated. The antibody significantly reduced the tumor volume compared to the control group. The effect was comparable to ERBITUX when human lung cells H1373 were used (US9388249B2).

Western Blot, Other Application

Research Use Only

Monoclonal

048-006

EGFR

epidermal growth factor receptor

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Rabbit

IgG

Epidermal growth factor receptor

Human

-20°C,2°C to 8°C

12352203