Western blot analysis of HIF2A using anti-HIF2A antibody (PA1129-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat testis tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF2A antigen affinity purified polyclonal antibody (Catalog # PA1129-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF2A at approximately 110-120KD. The expected band size for HIF2A is at 96KD.
Western blot analysis of HIF2A using anti-HIF2A antibody (PA1129-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat testis tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF2A antigen affinity purified polyclonal antibody (Catalog # PA1129-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF2A at approximately 110-120KD. The expected band size for HIF2A is at 96KD.
Anti-HIF-2-alpha/EPAS1 Antibody Picoband(r)
PA1129-2
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetEpas1
Overview
- SupplierBoster Bio
- Product NameAnti-HIF-2-Alpha Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID29452
- Target nameEpas1
- Target descriptionendothelial PAS domain protein 1
- Target synonymsendothelial PAS domain-containing protein 1; EPAS-1; HIF2 alpha; HIF-2 alpha; Hif2a; HIF-2-alpha; HIF2-alpha; HLF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia-inducible factor 2-alpha
- HostRabbit
- IsotypeIgG
- Protein IDQ9JHS1
- Protein NameEndothelial PAS domain-containing protein 1
- Scientific DescriptionBoster Bio Anti-HIF-2-alpha/EPAS1 Antibody catalog # PA1129-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203