Anti-hINSR [IR 83-22]

Absolute Antibody

Anti-hINSR [IR 83-22]

7

This antibody is specific for human INSR, reacting with an epitope in the alpha subunit (amino acids 485-592). It does not cross-react with rabbit, rat or mouse insulin receptors, and displays very weak cross-reactivity with bovine, porcine and ovine rece

This antibody has been used in immunoprecipitation assays, with both radiolabelled hINSR and hINSR-radiolabelled insulin complexes (Soos et al, 1986). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptors alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to IM-9 cells (Soos et al, 1986), and behaves as a surrogate agonist.

ImmunoPrecipitation, Western Blot, Neutralisation/Blocking

IP; IB; inhibitory

Research Use Only

Monoclonal

IR 83-22

INSR

insulin receptor

CD220; HHF5; insulin receptor; IR

Mouse

IgG1

Insulin receptor

Human

Human

This antibody was raised by immunising BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental insulin receptor prior to fusion.

-20°C,2°C to 8°C

12352203