Bio-Connect

Anti-His tag (C-term) [3D5]

Ab00101-12.1
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-His tag (C-term) [3D5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301).
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Scientific Description
    This is a chimeric human antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203