Anti-His tag (C-term) [3D5]

Absolute Antibody

Anti-His tag (C-term) [3D5]

9

This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301).

Flow Cytometry, Western Blot, ELISA

Research Use Only

Monoclonal

3D5

Rat

IgG1

This is a chimeric rat antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203