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ProductsBack/anti-His-Tag Chimeric Human, Rabbit Monoclonal (RMH01)
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antib
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antib
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antib

anti-His-Tag Chimeric Human, Rabbit Monoclonal (RMH01)

Research Use Only
REV-54-1161-00
RevMAb Biosciences
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
ReactivityAll Species
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-His-Tag Chimeric Human, Rabbit Monoclonal (RMH01)
  • Delivery Days Customer
    5
  • Antibody Specificity
    This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The anti
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RMH01
  • Concentration
    1 mg/ml
  • Formulation
    Liquid
  • Host
    Human
  • Isotype
    IgG
  • Scientific Description
    His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. - Recombinant Antibody. This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody. Applications: WB, ELISA. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
  • Reactivity
    All Species
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203