Figure 1. Western blot analysis of HDAC2 using anti-HDAC2 antibody (PA1350). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brian tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 antigen affinity purified polyclonal antibody (Catalog # PA1350) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 55 kDa.
Figure 1. Western blot analysis of HDAC2 using anti-HDAC2 antibody (PA1350). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brian tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 antigen affinity purified polyclonal antibody (Catalog # PA1350) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 55 kDa.
Anti-Histone deacetylase 2 HDAC2 Antibody Picoband(r)
PA1350-APC
ApplicationsWestern Blot
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetHDAC2
Overview
- SupplierBoster Bio
- Product NameAnti-Histone deacetylase 2 HDAC2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateAPC (Allophycocyanin)
- Gene ID3066
- Target nameHDAC2
- Target descriptionhistone deacetylase 2
- Target synonymsHD2; histone deacetylase 2; KDAC2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1
- HostRabbit
- IsotypeIgG
- Protein IDQ92769
- Protein NameHistone deacetylase 2
- Scientific DescriptionBoster Bio Anti-Histone deacetylase 2 HDAC2 Antibody catalog # PA1350. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityChicken, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203