Anti-HLA-A2 [MBW1]

Absolute Antibody

Anti-HLA-A2 [MBW1]

7

This antibody specifically reacts with human HLA-A2 and does not cross react with A28, Aw68, or Aw69 antigens (PMID: 2160447). The reactivity of this antibody to human HLA-A2 was determined using ELISA. The reactivity of this antibody towards HLA-A2 was abolished by substitutions in the a1 domain, specifically the substitutions of alanine for glutamic acid at position 58 (E58A) and either a valine or arginine substitution for glycine at position 62 (G62V or G62R). The reactivity of this antibody to surface bound HLA-A*0201 was determined using complement-dependent cytotoxicity (CDC) assay (PMID: 9862700).

Functional Assay, ELISA

ELISA; functional assay

Research Use Only

Monoclonal

MBW1

HLA-A

major histocompatibility complex, class I, A

HLAA, HLA class I histocompatibility antigen, A alpha chain, HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen HLA-A heavy chain, leukocyte antigen class I-A

Human

IgG1

HLA class I histocompatibility antigen, A alpha chain

This reformatted human antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated from the peripheral blood lymphocytes obtained from a multiparous volunteer 1 week postpartum were transformed in vitro by Epstein-Barr virus and electro fused with the heterohybridoma fusion partner, K6H6/B5. The screening for specific clone was done using micro lymphocytotoxicity assay.

-20°C,2°C to 8°C

41116161