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ProductsBack/Anti-HLA-A2 [SN66E3]

Anti-HLA-A2 [SN66E3]

AB03372-10.0-BT
Absolute Antibody
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetHLA-A
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-HLA-A2 [SN66E3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody was used in the flow cytometric analysis of HLA class I antigen expression levels on Lymphoblastoid cell lines and class I deficient mutant cells (PMID: 9820600).
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SN66E3
  • Gene ID3105
  • Target name
    HLA-A
  • Target description
    major histocompatibility complex, class I, A
  • Target synonyms
    HLAA, HLA class I histocompatibility antigen, A alpha chain, HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen HLA-A heavy chain, leukocyte antigen class I-A
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP04439
  • Protein Name
    HLA class I histocompatibility antigen, A alpha chain
  • Scientific Description
    This reformatted human antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HLA A using anti-HLA A antibody (PB9376). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA A antigen affinity purified polyclonal antibody (Catalog # PB9376) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA A at approximately 41 kDa. The expected band size for HLA A is at 41 kDa.
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