Anti-HLA-A2/A28 [3PF12]

Absolute Antibody

Anti-HLA-A2/A28 [3PF12]

7

ScFv 3PF12 has been used in immunofluorescence and flow cytometry experiments to test antibody binding to HLA-A2 positive lymphocytes and platelets (Watkins 2000). The scFv has been tested in in vitro assays and have been shown to inhibit target cell killing (Watkins 2003).

Flow Cytometry, ImmunoFluorescence

IF; FC

Research Use Only

Monoclonal

3PF12

Human

This reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Human

A 57-year-old female presented with post-transfusion purpura 5 days following a blood transfusion. Serological studies with the sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were selected by panning.

-20°C,2°C to 8°C

12352203