Anti-HLA-DP Antibody

Biorbyt

Anti-HLA-DP Antibody

10

1. Take 100 microl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Flow Cytometry, ImmunoFluorescence

FC, IF

Research Use Only

Monoclonal

B7/21

Unconjugated

HLA-DPA1

major histocompatibility complex, class II, DP alpha 1

DP(W3); DP(W4); DPA1; HLA class II histocompatibility antigen, DP alpha 1 chain; HLA DPA1; HLADP; HLA-DP1A; HLA-DPA; HLA-DPA1*01:03:01:New; HLA-DPA1*02:01:01:NEW; HLA-DPB1; HLASB; HLA-SB alpha chain; MHC class II antigen DP alpha chain; MHC class II DP alpha chain; MHC class II DP3-alpha; MHC class II HLA-DPA1 antigen; MHC class II protein; PLT1

Mouse

HLA class II histocompatibility antigen, DP alpha 1 chain

Mouse monoclonal antibody to HLA-DP

Human

-20°C,2°C to 8°C

12352203