Anti-HLA-DR [HD8]

Absolute Antibody

Anti-HLA-DR [HD8]

7

This antibody specifically binds an epitope comprising amino acids 61-WNSQKDILEQARA-73 in the beta chain of human HLA-DR. This antibody can recognize most polymorphic forms of the human HLA-DR. This antibody can recognize 99.2% of HLA-DRB alleles and also

The binding specificity of this antibody to HLA-DR-positive B-lymphoblastoid cell line, SKW6.4 was tested using western blot. The reactivity of this antibody towards various HLA-DR positive cell lines like ARH77, Daudi, Granta519, IM-9, MC/CAR, Raji, RPMI1788, HS-Sultan, Namalwa, RL, Ramos, SKW6.4 etc. was determined using Alexa488 labelled antibody by flow cytometry. This antibody is reported to exert strong antibody dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro, and significantly extended the life span of immunocompromised mice inoculated with non-Hodgkin lymphoma cell lines. This antibody has potential applications in HLA-DR targeted immunotherapy as it is likely to evoke similarly strong responses in individuals carrying different HLA-DR alleles (PMID: 17428256). The Pro331Ser and Lys322Ala modified version of this antibody was incapable of expressing CDC in vitro and did not induce severe infusion reactions in rats and monkeys, even at extremely high doses. This antibody still retained its Ab-dependent cellular cytotoxicity function as well as its antitumor activity in a tumor-bearing mouse model (PMID: 18250438).

Flow Cytometry, Western Blot

WB; FC; immunotherapy

Research Use Only

Monoclonal

HD8

CD74

CD74 molecule

CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DHLAG; gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia-associated invariant chain; Ia-GAMMA; II; MHC HLA-DR gamma chain; p33

Rabbit

IgG

HLA class II histocompatibility antigen gamma chain

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG2 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing a human transgenic KM mice with HLA-DR-expressing L929 cells.

-20°C,2°C to 8°C

12352203