Anti-HLA-G [YWHG-5]

Absolute Antibody

Anti-HLA-G [YWHG-5]

9

This antibody specifically binds the amino acid sequence EGLPEPLMLRWSKEGDGGIMS in the alpha3 structural domain of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules

This antibody can bind human HLA-G peptide EGLPEPLMLRWSKEGDGGIMS in an ELISA with a binding affinity of Ka= 1.01 X 10^(9) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody was also used in the detection of soluble HLA-G5 and HLA-G6 using intracellular flow cytometry of K562-HLA-G5 and K562-HLA-G6 cells. This antibody can also be used for the immunohistochemical staining of HLA-G5/6 molecular expression in gastric cancer tissue using immunohistochemistry (CN114605543).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

YWHG-5

HLA-G

major histocompatibility complex, class I, G

b2 microglobulin; HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; MHC class Ib antigen; MHC-G; mutant MHC class I antigen; mutant MHC class Ib antigen

Rabbit

IgG

HLA class I histocompatibility antigen, alpha chain G

Human

-20°C,2°C to 8°C

12352203