Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody(M00066-1) HMGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HMGB1 Antibody (M00066-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody(M00066-1) HMGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HMGB1 Antibody (M00066-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody
M00066-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHMGB1
Overview
- SupplierBoster Bio
- Product NameAnti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAAC-8
- FormulationLiquid
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsAmphoterin; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; HMG-1; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP09429
- Protein NameHigh mobility group protein B1
- Scientific DescriptionBoster Bio Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody catalog # M00066-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Short-term pretreatment of naringin isolated from Citrus wilsonii Tanaka attenuates rat myocardial ischemia/reperfusion injury. Liu W et al., 2022 Sep, Naunyn Schmiedebergs Arch PharmacolRead more
- Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway. Huang SQ et al., 2021 Mar 7, World J GastroenterolRead more
- Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress. Wen Y et al., 2020 Jan 7, World J GastroenterolRead more