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IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-Hsc70 Antibody

Research Use Only
PA1214
Boster Bio
Product group Antibodies
Price on request
100 ug/vial
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Hsc70 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    IHP, IHF, ICC, WB, IHC
  • Certification
    Research Use Only
  • Concentration
    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
  • Estimated Purity
    Immunogen affinity purified.
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • UNSPSC
    12352203