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ProductsBack/Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband(r)
Figure 1. Western blot analysis of ST13 using anti-ST13 antibody (PA1935). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SKOV3 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # PA1935) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.
Figure 1. Western blot analysis of ST13 using anti-ST13 antibody (PA1935). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SKOV3 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # PA1935) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.
Figure 1. Western blot analysis of ST13 using anti-ST13 antibody (PA1935). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SKOV3 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # PA1935) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.

Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband(r)

Research Use Only
PA1935-DYLIGHT594
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHamster, Human
TargetST13
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID6767
  • Target name
    ST13
  • Target description
    ST13 Hsp70 interacting protein
  • Target synonyms
    AAG2; aging-associated protein 2; FAM10A1; FAM10A4; heat shock 70kD protein binding protein; HIP; HOP; hsc70-interacting protein; Hsp70-interacting protein; HSPABP; HSPABP1; P48; PRO0786; progesterone receptor-associated p48 protein; putative tumor suppressor ST13; renal carcinoma antigen NY-REN-33; SNC6; suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein); suppression of tumorigenicity 13 protein; testis secretory sperm-binding protein Li 233m
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50502
  • Protein Name
    Hsc70-interacting protein
  • Scientific Description
    Boster Bio Anti-HSC70 Interacting Protein HIP/ST13 Antibody catalog # PA1935. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Hamster, Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203