IHC analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). Hsp70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Hsp70 Antibody (M00949-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
IHC analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). Hsp70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Hsp70 Antibody (M00949-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-Hsp70 HSPA1A Antibody Picoband(r) (monoclonal, 3H5)
M00949-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSPA1A
Overview
- SupplierBoster Bio
- Product NameAnti-Hsp70 Antibody (monoclonal, 3H5)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3H5
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- Scientific DescriptionBoster Bio Anti-Hsp70 HSPA1A Antibody Picoband® (monoclonal, 3H5) catalog # M00949-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203