Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody (M00949) HSPA1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA1A Antibody (M00949)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody (M00949) HSPA1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA1A Antibody (M00949)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody
M00949
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPA1A
Overview
- SupplierBoster Bio
- Product NameAnti-Hsp70 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDDGE-8
- FormulationLiquid
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostRabbit
- IsotypeIgG
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- Scientific DescriptionBoster Bio Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody catalog # M00949. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Differentially expressed microRNA profiles in exosomes from vascular smooth muscle cells associated with coronary artery calcification. Pan W et al., 2020 Jan, Int J Biochem Cell BiolRead more