Anti-HSV-1 VP5 [6F10-D2-G2]

Absolute Antibody

Anti-HSV-1 VP5 [6F10-D2-G2]

9

This antibody is specific for residues 862-880 of the major capsid protein. The major capsid protein self-assembles to form an icosahedral capsid with a T=16 symmetry, about 200 nm in diameter, and consisting of 150 hexons and 12 pentons (total of 162 cap

To research the capsid of HSV-1, the mouse version of this antibody was used to immunoprecipitate the capsid of HSV-1 and characterized using ELISA on HSV-1 capsids (Newcomb et al., 1996; PMID: 8918599). To assess the structure of the herpes simplex virus capsid, proteins from BHK-21 cells infected with HSV-1 were immunoblotted with the mouse version of this antibody. Furthermore, the proteins from the BHK-21 cells infected with HSV-1 were immunoprecipitated with the mouse version of this antibody. The immunoprecipitated capsids bound to the mouse version of this antibody were then inspected using cryoelectron microscopy (Spencer et al., 1997; PMID: 9123829). While characterizing the mechanism of HSV-1 nucleocapsid egress, the mouse version of this antibody was used for immunofluorescence on Vero cells transfected with chimeric EGFP and/or infected with HSV-1 (Guan et al., 2014; PMID: 25036476).

Functional Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA

Research Use Only

Monoclonal

6F10-D2-G2

Mouse

IgG1

Virus

-20°C,2°C to 8°C

12352203