Anti-HTT-N17 [scFv-C4], scFv fragment (His), ScFv,

Absolute Antibody

Anti-HTT-N17 [scFv-C4], scFv fragment (His), ScFv,

9

The antibody is specific for huntingtin. It binds to the first 17 residues of huntingtin [HTT(1-17)].

The original format of the antibody could inhibit the aggregation of mHTT-exon1 protein fragments in vitro. The crystal structure of the antibody in complex with HTT(1-17) was determined (De Genst et al., 2015; PMID: 25861763). The specificity of the antibody was confirmed by ELISA analysis. The original format of the antibody-targeted the HD-Q103-myc antigen to cell nuclei in COS-7 cells by immunofluorescence microscopy. The binding affinity of the antibody to HTT(1-17) was measured by surface plasmon resonance (Kd= 7.9 nM) (Lecerf et al., 2001; PMID: 11296304). The antibody selectively bound diffuse htt, but not aggregated htt, as shown by immunofluorescence analysis. Immunoprecipitation experiments in stably transfected, intrabody-expressing STHdhQ111 cells demonstrated that the antibody bound a small fraction of wild-type and mutant endogenous full-length htt (Miller et al., 2005; PMID: 15837560).

ImmunoFluorescence, ELISA, Other Application

Research Use Only

Monoclonal

scFv-C4

HTT

huntingtin

HD; huntingtin; huntington disease protein; IT15; LOMARS

Huntingtin

This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203