Anti-Human Carbonic Anhydrase IX [M75]

Absolute Antibody

Anti-Human Carbonic Anhydrase IX [M75]

9

This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IXs PG domain.

The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastorekova et al., 1992; PMID: 1312272). This antibodys epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Zavada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 microM (Kral et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009; PMID: 19538935).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

M75

CA9

carbonic anhydrase 9

CAIX; CA-IX; carbonate dehydratase IX; carbonic anhydrase 9; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; MN; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250

Human

IgG1

Carbonic anhydrase 9

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203