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Anti-Human Cytokeratin 8 [1E8-C6-B4]

Research Use Only
Ab02687-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetKRT8
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Human Cytokeratin 8 [1E8-C6-B4]
  • Delivery Days Customer
    7
  • Antibody Specificity
    The antibody is specific for the C-terminus of cytokeratin 8, one member of a diverse group of proteins called intermediate filament proteins. These proteins are often expressed in pairs. Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the
  • Application Supplier Note
    The original antibody detected CK 8 by western blot from BT20 breast cancer cells. Immunofluorescence was performed on BT20 cells using this antibody, showing the carboxyl terminus of CK 8 was exposed on the surfaces of breast cancer cells. The Fab fragment of the antibody was used for inhibition experiments on breast cancer cell lines BT20, MDA-MB-157, MCF-7 and purified CK 8. Results showed a decreased plasminogen binding in all the experiments. The Fab fragment was used to study the role of cell-surface CK 8 in promoting the activation of plasminogen by t-PA in MCF-7 cells; preincubation of MCF-7 cells with the Fab fragment caused a concentration-dependent decrease in the ability of the cell cultures to promote plasminogen activation (Hembrough et al, 1996; pmid:8810346).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Applications Supplier
    WB, IP, IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1E8-C6-B4
  • Gene ID3856
  • Target name
    KRT8
  • Target description
    keratin 8
  • Target synonyms
    CARD2; CK8; CK-8; CYK8; cytokeratin-8; K2C8; K8; keratin 8, type II; keratin, type II cytoskeletal 8; KO; type-II keratin Kb8
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05787
  • Protein Name
    Keratin, type II cytoskeletal 8
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing mice against the C-terminus (amino acids 472-483) of human Cytokeratin 8.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203