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Western blot analysis of Huntingtin expression in HeLa cell lysate.
Western blot analysis of Huntingtin expression in HeLa cell lysate.
Western blot analysis of Huntingtin expression in HeLa cell lysate.

Anti-Huntingtin Monoclonal Antibody

M00134
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
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Product group Antibodies
TargetHTT
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Huntingtin Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ADGC-8
  • Gene ID3064
  • Target name
    HTT
  • Target description
    huntingtin
  • Target synonyms
    HD; huntingtin; huntington disease protein; IT15; LOMARS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42858
  • Protein Name
    Huntingtin
  • Scientific Description
    Boster Bio Anti-Huntingtin Monoclonal Antibody catalog # M00134. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody (A00134-2). Huntingtin/HTT was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Huntingtin/HTT Antibody (A00134-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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