Anti-Hypodermis [KT13]

Absolute Antibody

Anti-Hypodermis [KT13]

7

This antibody recognizes fibrous structures in seam cells of hypodermis of Caenorhabditis elegans that are detectable only beginning from the pretzel stage. Caenorhabditis elegans is a transparent nematode that lives in temperate soil environments.

This antibody stained distinct subsets of hypodermal cells at specific periods during embryogenesis. This antibody was also used for staining of the seam cells of a pretzel stage embryo. This antibody recognized KT13 recognized the lateral hypodermis (called seam cells) at the pretzel stage, but not at earlier stages. Seam cells at the pretzel stage have a long rectangular shape, and the this antibody sends signals that were stronger along the edge of the long sides of the rectangle. With higher magnification, The KT13 signal appeared as parallel fibrous structures along the short axis of each seam cell (PMID:18498356).

ImmunoFluorescence

IF

Research Use Only

Monoclonal

KT13

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.

C. Elegans

Caenorhabditis elegans

The original antibody was generated by immunizing BALB/c mice with subtracted embryonic extract. Subtracted extracts were prepared by using a method called “antigen subtraction” which enriched antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens.

-20°C,2°C to 8°C

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