Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Anti-Interferon gamma/IFNG Antibody Picoband(r)
PB9024
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetIfng
Overview
- SupplierBoster Bio
- Product NameAnti-IFN gamma Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Interferon gamma is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- Applications SupplierELI, WB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID25712
- Target nameIfng
- Target descriptioninterferon gamma
- Target synonymsIf2f; IFNG2; IFN-gamma; interferon 2f; interferon gamma
- HostRabbit
- IsotypeIgG
- Protein IDP01581
- Protein NameInterferon gamma
- Scientific DescriptionBoster Bio Anti-Interferon gamma/IFNG Antibody Picoband® catalog # PB9024. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Reactivity SupplierRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203