Anti-IgE [R1E4]

Absolute Antibody

Anti-IgE [R1E4]

9

This antibody is specific for murine IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylati

In the original study, this antibody and other clones (C12B9, 23G3, and B1E3) of the monoclonal anti-mIgE antibodies were used in conjunction with the recombinant chimeric mIgE-human IgG1 molecules (Keegan et al., 1991). It has been demonstrated that the Cepsilon3 domain is important in the binding of mIgE to the murine B cell FcepsilonRII as well as to the murine mast cell FcepsilonRI (Keegan et al., 1991). Also, while the Cepsilon4 domain had no effect on binding to the FcepsilonRI, the presence of the Cepsilon4 domain influenced the binding of the recombinant IgE to the FcepsilonRII (Keegan et al., 1991). This antibody blocks IgE binding to mouse or rat FceRI and can be used in mouse IgE ELISA. In addition, this antibody could be used, for instance, to develop a flow cytometry assay for the identification and differentiation of chemicals with the potential to elicit irritation, IgE-mediated, or T cell-mediated hypersensitivity responses (Manetz et al, 1999), or to study the kinetics of the ligand-IgE interaction (Posner et al., 1992).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

R1E4

Rat

IgG1

Ig epsilon chain C region

Mouse

-20°C,2°C to 8°C

12352203