Anti-IGF-IR [7C10]

Absolute Antibody

Anti-IGF-IR [7C10]

9

The antibody is specific for human IGF-IR. It does not prevent clone 12BI from binding to the same protein. IGF-IR is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insu

Specificity of this antibody was confirmed by ELISA (WO2009017679). Further, western blot was preformed to test whether the antibody cross reacts with IR protein (WO2009017679). It was also observed that the human version of this antibody was useful for antibody dependent cell cytotoxicity assays; MCF-7 or A549 target cells were incubated with this antibody and human natural killer cells. It was shown that the antibody does increase lysis of these cells (Goetsch et al, 2005; pmid:15386423). Furthermore, it was shown that the human version of this antibody is able to inhibit growth of MDA-MB-231, an oestrogen-independent breast cancer cell line in mice (Pandini et al, 2007; pmid:17451939). The human version of this antibody was also used in immunofluorescence on MCF-7 cells (Broussas et al, 2009; pmid:19165858). Finally, human version of this antibody was utilized in western blot of MCF-7 cells tumors (Broussas et al, 2009; pmid:19165858).

Functional Assay, Western Blot, ELISA

Research Use Only

Monoclonal

7C10

IGF1R

insulin like growth factor 1 receptor

CD221; IGF-I receptor; IGFIR; IGFR; insulin-like growth factor 1 receptor; JTK13; soluble IGF1R variant 1; soluble IGF1R variant 2

Human

IgG1

Insulin-like growth factor 1 receptor

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203